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Title: Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi.
Authors: Lage, Ramon Silva
Oliveira, Guilherme Corrêa de
Busek, Solange Cristina Uber
Guerra, Luanda Liboreiro
Giunchetti, Rodolfo Cordeiro
Oliveira, Rodrigo Corrêa de
Reis, Alexandre Barbosa
Keywords: Cytokines
Canine disease
Visceral leishmaniasis
Leishmania chagasi
Issue Date: 2007
Citation: LAGE, R. S. et al. Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi. Veterinary Immunology and immunopathology, v. 115, n. 1-2, p. 135-145, jan. 2007. Disponível em: <>. Acesso em: 10 jul. 2012.
Abstract: Recent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL-10, IL-12, INF- g and TNF- a in splenocytes of 30 dogs naturally infected with Leishmania chagasiand of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 ( p = 0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher ( p = 0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL-10 was higher (p = 0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r = 0.3510; p = 0.0337) and the levels of expression of IL-10 and INF- g increase in parasitism (IL-10: r = 0.3428; p = 0.0438 and INF- g: r = 0.4690; p = 0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF- g was related with the increase in parasitic load.
ISSN: 01652427
metadata.dc.rights.license: O periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3303250782673.
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